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human dermal fibroblasts hdf cell line  (Innoprot Inc)


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    Structured Review

    Innoprot Inc human dermal fibroblasts hdf cell line
    Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal <t>fibroblasts</t> <t>(HDF)</t> after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
    Human Dermal Fibroblasts Hdf Cell Line, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+dermal+fibroblasts+hdfs/Human+Dermal+Fibroblasts/pmc13364450-106-19-26
    Average 93 stars, based on 80 article reviews
    human dermal fibroblasts hdf cell line - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Amylopectin- g -Poly(Acrylic Acid): Synthesis and Application as Reduction Agent for In Situ Formation of Gold Nanoparticles"

    Article Title: Amylopectin- g -Poly(Acrylic Acid): Synthesis and Application as Reduction Agent for In Situ Formation of Gold Nanoparticles

    Journal: Polymers

    doi: 10.3390/polym18131636

    Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal fibroblasts (HDF) after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).
    Figure Legend Snippet: Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal fibroblasts (HDF) after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).

    Techniques Used: Cell Viability Assay, Negative Control, Standard Deviation, Control

    Related Articles

    Cell Culture:

    Article Title: Innovative Processing and Sterilization Techniques to Unlock the Potential of Silk Sericin for Biomedical Applications
    Article Snippet: SS.L powder obtained by cryo-lyophilization (SS.L) and sterilized by scCO 2 was compared with a commercial SS powder (Sigma-Aldrich Merck, S5201, Darmstadt, Germany) to evaluate differences in the gelling properties and discuss the commercial value of the developed methodology. .. Cell culture assays were performed using human dermal fibroblasts (HDFs) (Innoprot, P10856). .. For the in vitro biological evaluation, cells were cultured in a basal medium consisting of Dulbecco’s modified Eagle’s medium (DMEM)—low glucose enriched with 110 mg/L of sodium bicarbonate and supplemented with 20% v : v of fetal bovine serum (FBS, Gibco Thermo Fisher, São Paulo, Brasil), 200 mM L-glutamine, 100 units/mL penicillin, and 100 mg/mL streptomycin.

    Article Title: Innovative Processing and Sterilization Techniques to Unlock the Potential of Silk Sericin for Biomedical Applications.
    Article Snippet: .. Biological Behavior Cell culture assays were performed using human dermal fibroblasts (HDFs) (Innoprot, P10856). .. For the in vitro biological evaluation, cells were cultured in a basal medium consisting of Dulbecco’s modified Eagle’s medium (DMEM)—low glucose enriched with 110 mg/L of sodium bicarbonate and supplemented with 20% v:v of fetal bovine serum (FBS, Gibco Thermo Fisher, São Paulo, Brasil), 200 mM L-glutamine, 100 units/mL penicillin, and 100 mg/mL streptomycin.

    other:

    Article Title: Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models.
    Article Snippet: Please cite this article as: Santo, Vítor E., Estrada, Marta F., Rebelo, Sofia P., Abreu, SofiaC., Silva, InêsM., Pinto, Catarina, Veloso, SusanaC., Serra, AnaTeresa, Boghaert, Erwin, Alves, Paula M., Brito, Catarina, Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models.Journal of Biotechnology http://dx.doi.org/10.1016/j.jbiotec.2016.01.031

    Modification:

    Article Title: Biofunctional silk sericin hydrogels: A versatile platform with potential for tissue healing and regeneration.
    Article Snippet: Discarded silk sericin protein (SS) presents a high yet underexplored potential as a biomaterial for tissue engineering (TE).. Despite its biocompatibility, antioxidant activity, and moisture retention properties, its poor stability in aqueous media has limited broader application.. In this work, we developed and characterized SS-based hydrogels using tannic acid (TA) and horseradish peroxidase (HRP) crosslinking systems to address these limitations and expand their use in skin TE.

    Article Title: Continuous Production of Highly Tuned Silk/Calcium-Based Composites: Exploring New Pathways for Skin Regeneration
    Article Snippet: Finally, for particle size distribution analysis, suspensions were collected at the end of each experiment and directly analyzed by laser diffraction using ethanol as a solvent (LS 230, Beckman Coulter, Brea, CA, USA). .. Human Dermal Fibroblasts (HDFs) obtained from Innoprot (Ref: P10856-7-8) were grown as a monolayer under standard conditions (37 °C, 5% CO 2 ) in basal medium consisting of Dulbecco’s Modified Eagle’s Medium (DMEM-Dulbecco’s Modified Eagle Medium; GibcoTM DMEM, high glucose), supplemented with 10% fetal bovine serum (FBS; BioWest), and 1% of penicillin-streptomycin solution (Lonza, Basel, Switzerland). .. At confluence, cells were detached from the culture flasks using TrypLE Express (Gibco, Waltham, MA USA), centrifuged, resuspended in culture medium, and seeded at a density of 1 × 10 4 cells per cm 2 .

    Isolation:

    Article Title: A computational diffusion model to study antibody transport within reconstructed tumor microenvironments
    Article Snippet: NCI-H157 (#CRL-5802; from now on referred as H157) Non-Small Cell Lung Carcinoma (NSCLC) cell line was obtained from American Type Culture Collection (ATCC). .. Human Dermal Fibroblasts (hDFs) isolated from human skin were obtained from Innoprot. .. Cell were routinely screened for mycoplasma contamination by PCR (Eurofins Genomics Europe Applied Genomics GmbH, Germany).



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    Image Search Results


    Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal fibroblasts (HDF) after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).

    Journal: Polymers

    Article Title: Amylopectin- g -Poly(Acrylic Acid): Synthesis and Application as Reduction Agent for In Situ Formation of Gold Nanoparticles

    doi: 10.3390/polym18131636

    Figure Lengend Snippet: Cytotoxicity assessment of AMP- g -PAA and AMP- g -PAA/AuNPs in human dermal fibroblasts (HDF) after 48 h exposure. Relative cell viability (%) of HDF cells treated with increasing concentrations (0.125, 0.25, 0.5, and 1 µg/mL) of AMP- g -PAA (dotted light grey bars) and AMP- g -PAA/AuNPs (solid dark grey bars), as determined by the CellTiter-Glo ® Luminescent Cell Viability Assay. Cell viability was normalized to the untreated negative control and measured by ATP-dependent bioluminescence. Numerical values inside each bar indicate mean relative cell viability (%). Data are expressed as mean ± standard deviation from three independent experiments performed in duplicate. Statistical significance was assessed by two-way ANOVA, followed by Dunnett’s multiple comparisons test against the untreated control. ns = not significant ( p > 0.05).

    Article Snippet: The cytotoxicity of both the AMP- g -PAA copolymer and the AMP- g -PAA/AuNPs composites was evaluated using a Human dermal fibroblasts (HDF) cell line from Innoprot (Derio, Spain).

    Techniques: Cell Viability Assay, Negative Control, Standard Deviation, Control